Loading...

Immunoelectrophoresis

. 52 Videos
Subscribe
22,548,145
2,729,347 views
95% 39,852 852

About :

For more information, log on to-
http://shomusbiology.weebly.com/
Download the study materials here-
http://shomusbiology.weebly.com/bio-materials.html
Source of all articles published in description is Wikipedia. Thanks to original content developers.
Link- http://en.wikipedia.org/wiki/Main_Page
Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. All variants of immunoelectrophoresis require immunoglobulins, also known as antibodies reacting with the proteins to be separated or characterized. The methods were developed and used extensively during the second half of the 20th century. In somewhat chronological order: Immunoelectrophoretic analysis (one-dimensional immunoelectrophoresis ad modum Grabar), crossed immunoelectrophoresis (two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ad modum Laurell), rocket-immunoelectrophoresis (one-dimensional quantitative immunoelectrophoresis ad modum Laurell), fused rocket immunoelectrophoresis ad modum Svendsen and Harboe, affinity immunoelectrophoresis ad modum Bøg-Hansen.

Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for the electrophoresis as well as the reaction with antibodies. The agarose was chosen as the gel matrix because it has large pores allowing free passage and separation of proteins, but provides an anchor for the immunoprecipitates of protein and specific antibodies. The high pH was chosen because antibodies are practically immobile at high pH. An electrophoresis equipment with a horizontal cooling plate was normally recommended for the electrophoresis.

Immunoprecipitates may be seen in the wet agarose gel, but are stained with protein stains like Coomassie Brilliant Blue in the dried gel. In contrast to SDS-gel electrophoresis, the electrophoresis in agarose allows native conditions, preserving the native structure and activities of the proteins under investigation, therefore immunoelectrophoresis allows characterization of enzyme activities and ligand binding etc. in addition to electrophoretic separation.

The immunoelectrophoretic analysis ad modum Grabar is the classical method of immunoelectrophoresis. Proteins are separated by electrophoresis, then antibodies are applied in a trough next to the separated proteins and immunoprecipitates are formed after a period of diffusion of the separated proteins and antibodies against each other. The introduction of the immunoelectrophoretic analysis gave a great boost to protein chemistry, some of the very first results were the resolution of proteins in biological fluids and biological extracts. Among the important observations made were the great number of different proteins in serum, the existence of several immunoglobulin classes and their electrophoretic heterogeneity.

painel de vidro minecraft

atualizar smart hub samsung

como colocar optifine

como tirar o modo de seguranca do celular samsung duos

traducao de sorry justin bieber

corel x7 nao salva nem exporta

como jogar black no pc

licenca avast free antivirus ate 2038

nox nao instala

out of memory crossfire

Tags :

biology education teaching life sciences molecular biology cell biology suman bhattacharjee shomu's biology DNA proteins immunoelectrophoresis antibodies antigens electrophoresis affinity electrophoresis antigen antibody complex antigens and antibodies crossed immunoelectrophoresis agarose electroblotting

Google AdSense
728 x 90

Similar Videos:

50 Comments

    Popular Comments:

Anitha Polamuri . 2020-01-10
Thank you,,now i understand this topic,,
121 2 . Reply
Sakthi Vignesh Maharajan . 2019-12-23
Could you explain about rocket immunoelectrophoresis
121 2 . Reply
Monika dwivedi . 2019-12-19
Keep it up ....amazing explanation....just need to watch ur video once and the concept is clear😄
121 2 . Reply
pretty bean . 2019-11-18
Is electrophoresis a 2d gel electrophoresis?
121 2 . Reply
Radhika Mittal . 2019-11-09
Try to make all of ur vedioes in both Hindi and English bcoz I really appreciate ur vedioes..and they really helped me alot
121 2 . Reply
Ahmad Hani Morad . 2019-08-14
THank u very much . I finally understand it completely. ^__^
121 2 . Reply
vivre paisiblement c est tous qui compte les gens . 2019-07-04
Thanks
121 2 . Reply
The light to medicine Dr MSR . 2019-04-29
Thanks sir
121 2 . Reply
prashant sagar . 2019-04-24
Thank you sir ,it is easy to understand by your presentation ....
121 2 . Reply
Jay Pawar . 2018-08-22
sir i want to know about rocket electrophoresis
121 2 . Reply
muntaha bajwa . 2018-06-02
i mean wow
121 2 . Reply
prince . 2018-05-13
sound quality is very irritating nothing is visible clear
121 2 . Reply
Chotu Sahu . 2018-05-03
Nice lecture
121 2 . Reply
Chotu Sahu . 2018-05-03
Thankq sir
121 2 . Reply
APEKSHA SINGH . 2018-03-10
Useful
Please can uh upload on Immunonephelometry
121 2 . Reply
Prajkta Jadhav . 2017-12-10
U r amazing sir
121 2 . Reply
kit kat . 2017-12-01
Really it was helpful thnx sir
121 2 . Reply
Tanya Yadav . 2017-11-26
Thanku so much sir
121 2 . Reply
Nehemiah aka Neo . 2017-09-07
You are the awesomest
121 2 . Reply
Sriradha Ganguli . 2017-07-06
very helpful video Sir
121 2 . Reply
anulal k . 2017-06-08
thanks 4 the vedio. if immune electrophoresis and immuno electrophoresis are same?
121 2 . Reply
je suis algérienne . 2017-04-22
merci pour aide me dans mon exposé😃
121 2 . Reply
SS gearup . 2017-03-05
well done sir
121 2 . Reply
Danielle Vasconcelos . 2017-02-22
Very good video. Congratulations from Brazil!
121 2 . Reply
Muayad Mohammed . 2017-01-14
Thank you very much that was very much helpful
121 2 . Reply
Bharath Kumar Nandavaram . 2017-01-10
Tqq fr such a clear explanation
121 2 . Reply
Balveer . 2017-01-09
👍
121 2 . Reply
Gurminder Singh . 2016-12-04
Which book would you refer for antigen antibody interactions.?
121 2 . Reply
Sarwat Farooq . 2016-11-05
thank u!
121 2 . Reply
Seung-Heon Lee . 2016-11-02
You were quite helpful. I liked your way of explaining the importance of the precipitation line!
121 2 . Reply
smile relax . 2016-09-18
thanks a lot .very easy to understand..i am an Msc student..very useful lecture
121 2 . Reply
Himani Goel . 2016-06-06
hey, thanx fr such video it was really helpful to me..BT I HV a question that if that Ab do nt react with the Ag than. wt will happen?
121 2 . Reply
Purvanshi Vakil . 2016-04-05
Can u please make a video on agglutination inhibition
121 2 . Reply
SHIMWA Constantin . 2016-03-28
very nice
121 2 . Reply
Siham SAIDI . 2016-03-10
Thank uuuuu
121 2 . Reply
Samuel cuñat . 2016-01-17
This was very usefull for me., Thanks. Congratulations from Spain.
121 2 . Reply
bruna nathaly . 2016-01-16
thank you :)
121 2 . Reply
Mohammad Al-AZAZMEH . 2015-12-31
very nice thanks
121 2 . Reply
Essy Ely . 2015-12-16
Thanks alot, great video and easy to understand.
121 2 . Reply
Nishka Shah . 2015-10-12
great video!
121 2 . Reply
isabella gracia . 2015-10-04
Thanks a lot this is so helpful!
121 2 . Reply
Judy Oranika . 2015-09-28
Thanks!!!
121 2 . Reply
AJ Prashad . 2015-08-19
Great videos . I thought this could also be used quantitatively because the height of each precipitin line is directly proportional to the amount of antigen added. if you use a few standards you can create a calibration curve to determine amount of the unknown . Would appreciate some clarification . Thanks !
121 2 . Reply
Essay Kim . 2015-07-21
Thanks much!
121 2 . Reply
אלמוג almog1212 . 2015-05-16
thank you:) you were great!!

very very helpfull!!
121 2 . Reply
Anuradha Kaushlendra . 2015-04-15
thanks alot
121 2 . Reply
Joey Orlando . 2015-02-24
4:15 - electrophoresis is not a separation technique based on charge, it's based on size 
121 2 . Reply
Samsadist . 2015-01-18
thanx ! ;D
121 2 . Reply
Erzsébet Hollandus . 2014-07-01
I have problem only on " read"the small plates 
121 2 . Reply
sterrevv . 2014-05-31
REALLY HELPFUL! saved my day thank you thank you 
121 2 . Reply
Radial immunodiffusion
15:19
2,729,347 views
55%
ELISA - in simple steps
15:19
2,729,347 views
55%
Flow Cytometry
15:19
2,729,347 views
55%
What is a Protein?
15:19
2,729,347 views
55%
Hemagglutination assay
15:19
2,729,347 views
55%
Immunofluorescence
15:19
2,729,347 views
55%
Immunoprecipitation
15:19
2,729,347 views
55%
Western Blotting
15:19
2,729,347 views
55%
Native gel electrophoresis
15:19
2,729,347 views
55%
Google AdSense
336 x 280