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Rocket Immuno Electrophoresis

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RIEP known as electro immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen in an unknown sample. Various concentrations of antigens are loaded. On electrophoresis, the antigen begins to migrate towards anode. Antigen and antibody interacts, when it reaches the equivalence point, Ag-Ab complex precipitates. The precipitation line is seen in the form of a rocket.

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Agricultural Tamil Nadu Agricultural University (Organization) lab Tamil Nadu Agricultural University University TNAUgenomic Tnaugenomics Rocket Immuno Electrophoresis TNAU genomics immuno diffusion laboratory

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50 Comments

    Popular Comments:

Jyoti Soni . 2019-06-26
Why we did used moist chamber for incubation?
121 2 . Reply
Aladdin Creations . 2019-06-08
Thank you , good video
121 2 . Reply
Saubhik Ghosh . 2019-02-07
Where is Ab?
121 2 . Reply
Jaya Chendrayan . 2018-10-07
Sir,can we use PAGE techniques insted of this?
121 2 . Reply
Abdullatheef i like shin chan . 2018-10-06
👍👌
121 2 . Reply
Mohamad Azhan . 2018-01-20
This is easy to understand. Thanks for this great work!
121 2 . Reply
Venkatesh Murugasan . 2016-07-02
you really need to update yourself. we don't need to make wells manually. we can use a comb which is available in the market to make gel wells with appropriate size
121 2 . Reply
Gusha . 2016-06-17
no gloves, I r8 A-
121 2 . Reply
ShaMels . 2014-08-24
when they melt in the microwave it usually just gets in a liquid state, and they when it cools I guess they add the Antibody. And I think they also use a tampon on the antigen and PBS.
121 2 . Reply
Chun-Chen Yao . 2014-04-15
Good video! Thanks a lot. But I wonder whether the antibody would get denatured while put into the gel because the gel is usually pretty hot, right? Is there any other treatment to prevent this?
121 2 . Reply
ramya raj . 2014-02-04
superb
121 2 . Reply
prince philip . 2013-11-23
thanks for the practical!!
121 2 . Reply
kanjeable . 2013-10-21
well explained
121 2 . Reply
Valarmathi Sri . 2013-09-23
nice ............
121 2 . Reply
Priyanka Palaniswamy . 2013-09-21
nice video.
121 2 . Reply
Rekha Kalangiyam . 2013-09-20
nice video
121 2 . Reply
Logeshwari Pichandi . 2013-09-19
nice video
121 2 . Reply
Abinaya Sree . 2013-09-19
well explained
121 2 . Reply
karunyah dharshini . 2013-09-19
clearly explained
121 2 . Reply
Nandha Kumar . 2013-09-19
well explained video...
121 2 . Reply
Gayathri Mani . 2013-09-19
nice one
121 2 . Reply
Illakeya Rathnam . 2013-09-19
gud job..well explained
121 2 . Reply
Godwin James . 2013-09-19
nice video....
121 2 . Reply
Pradeep P . 2013-09-19
nice video
121 2 . Reply
Jintu Vijayan . 2013-09-19
informative video..
121 2 . Reply
Essakkirani Murugan . 2013-09-19
well explained
121 2 . Reply
abarna kirubalani . 2013-09-19
informative video
121 2 . Reply
Pavithra N S . 2013-09-19
Good work..well explained
121 2 . Reply
Poonkodi Bharathi . 2013-09-19
nice video
121 2 . Reply
Taraka Chandrasekar . 2013-09-19
nice video .gud explaination
121 2 . Reply
Srividya Narasimhan . 2013-09-19
good explanation about rocket immunoelectrophoresis
121 2 . Reply
Abirami Bose . 2013-09-19
nice video
121 2 . Reply
muttharasi Kavitha . 2013-09-19
well explained video...
121 2 . Reply
Sountharya Palanivel . 2013-09-19
informative video...................
121 2 . Reply
krishna kumar . 2013-09-19
Nice video and what are the other diseases which can be diagonised?
121 2 . Reply
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